In this guest editorial, the author first discusses how gender is a historical and biopsychosocial construct. This means that there are many aspects of gender besides identity, such as gender expressions, roles and experiences. They address how this issue highlights some of these aspects but that these are not exhaustive, given the topic. They highlight how being able to consider our own gender identities, roles, expressions and experiences is an essential starting point if we are to be competent health-care providers. Finally, they suggest that stories might be one way to learn about aspects of gender we may not be as familiar with, as they allow us to connect emotionally, not just cognitively, with these aspects. © Copyright 2020 Creative Health Care Management.This essay speaks to the legacy value of nurses' caring for all people, no matter how they feel about the person's values or lifestyle, including the current issues around gender identity and sexual orientation. This legacy is deeply imbedded in the moral ethics of nursing and supports the proposition that if there isn't caring, it isn't nursing. © Copyright 2020 Creative Health Care Management.This article asks what the reasons are for the frequent linking of the image of the Holocaust with that of dementia in contemporary discursive and representational practice. In doing so, it analyses some of the numerous 21st-century examples of fiction, drama and film in which the figure of a Holocaust survivor living with dementia takes centre stage. It explores the contradictory cultural effects that arise from making such a connection, in contexts that include expressions of fear at the spectacle of dementia, as well as comparisons between the person living with that condition and the inmate of a concentration camp. Detailed consideration of novels by Jillian Cantor and Harriet Scott Chessman as well as a play by Michel Wallenstein and a film by Josh Appignanesi suggests that the fictions of this kind can appear to provide solace for the impending loss of the eyewitness generation, yet also offer potential for a model for caregiving practice to those living with dementia in broader terms. © Author(s) (or their employer(s)) 2020. No commercial re-use. See rights and permissions. Published by BMJ.Sterility testing of cellular therapy products along with the associated environmental monitoring requirements for aseptic facilities, including compounding pharmacies, continues to impact clinical microbiology laboratories as evidenced by the numerous discussions recurring on American Society for Microbiology DivC and ClinMicroNet listservs. This mini review provides an overview of this complex field of current good manufacturing practices (cGMP) based on biopharmaceutical industry standards, and will summarize the compendial and alternative rapid microbial test methods available for product sterility and Mycoplasma testing. In addition, this mini review highlights major overarching regulatory requirements governing any laboratory performing product testing as regulated by the United States Food and Drug Administration (FDA). These requirements are different to the clinical familiarities of Clinical Laboratory Improvement Act of 1988 (CLIA '88), the College of American Pathologists (CAP), and the Joint Commission on Accreditation of Healthcare Organizations (JCAHO) - all of which have no jurisdiction in this area. As the cellular therapy field continues to advance and an increasing number of medical centers participate in clinical trials of these novel therapies, it is critically important that laboratories have a sound understanding of the major regulations and cGMP practices governing microbiological testing in the biopharmaceutical industry. Copyright © 2020 American Society for Microbiology.Risk of mother-to-child transmission of Toxoplasma gondii (T. gondii) during pregnancy is much greater in women who are exposed to primary T. gondii infection (toxoplasmosis) after conception compared to those who exposed to the infection before conception. Therefore, laboratory tests that help classify recent primary toxoplasmosis are important tools for the management of pregnant women suspected to T. gondii exposure. Detection of Toxoplasma IgM (Toxo IgM) is a sensitive indicator of primary toxoplasmosis, but the indicator specificity is low because sometimes natural IgM antibodies react with Toxoplasma antigens in absence of the infection. Furthermore, Toxo IgM sometimes persists in blood serum for several months or years following the primary infection. In recent decades, Toxo IgG avidity assay has been used as a standard diagnostic technique for a better estimation of the infection acquisition time and identification of the primary T. gondii infection during pregnancy. Avidity is described as the aggregs; and g) current issues and controversies in diagnosis of primary T. gondii infections in pregnancy. Copyright © 2020 American Society for Microbiology.Data from a large prospective multicenter clinical validation study of a nucleic acid amplification in vitro diagnostic test for Mycoplasma genitalium were analyzed to describe the prevalence of M. genitalium infection, risk factors, and disease associations in female and male patients seeking care in diverse geographic regions of the United States. Among 1737 female and 1563 male participants, overall M. genitalium prevalence was ?10%, and was significantly higher in persons ages 15-24 compared to ages 35-39 (female 19.8% vs. 4.7%, OR 5.05 [95%CI3.01-8.46]; male 16.5% vs. 9.4%, OR 1.91 [95%CI1.20-3.02]). The risk for M. genitalium infection was higher in black (female 12.0% vs. 6.8%, OR 1.88 [95%CI 1.30-2.72]; male 12.9% vs. 6.9%, OR 2.02 [95%CI1.38-2.96]) and non-Hispanic (female 11.2% vs. 6.0%, OR 1.97 [95%CI 1.25-3.10]; male 11.6% vs. 6.8%, OR 1.80 [95%CI1.14-2.85]) study participants. https://www.selleckchem.com/products/lys05.html Participants reporting urogenital symptoms had significantly elevated risk of M. genitalium infection compared to asymptomatic individuals (female OR 1.53 [95%CI 1.09-2.14]; male OR 1.42 [95%CI1.02-1.99]). Women diagnosed with vaginitis and cervicitis had higher prevalence of M. genitalium than women without those diagnoses, although this was statistically significant only for vaginitis (vaginitis OR 1.88 [95%CI 1.37-2.58]; cervicitis OR 1.42 [95%CI 0.61-2.96]). A diagnosis of urethritis in men was also significantly associated with M. genitalium infection (OR 2.97 [95%CI 2.14-4.13]). Few characteristics distinguished asymptomatic from symptomatic M. genitalium infections These results from persons seeking care in the United States, suggest that M. genitalium infection should be considered in young persons presenting with urogenital symptoms. Copyright © 2020 Manhart et al.